Evaluating Amperia for AAV Quantification at NHSBT: Strong Correlation with Automated ELISA

The Clinical Biotechnology Centre (CBC) at NHS Blood and Transplant (NHSBT) evaluated Amperia RED for AAV2 titre analysis during process development. Results showed strong correlation with their existing automated ELISA system (R² = 0.97).

In this case study, we share how Amperia was used to quantify AAV2 across multiple process steps without batching.

The team also reported faster prep, easier handling, and improved flexibility in sample throughput.

High Accuracy Across Process Steps

• Strong correlation with automated ELISA (R² = 0.97)

• Reliable titre measurement at harvest, clarification, filtration, and purification

• Maintains performance even with high-titre samples beyond ELISA’s range

Faster, More Flexible Workflows

• No batching required — run only the samples you need

• Lower dilution requirements and simpler prep

• Supports quicker optimisation cycles and real-time troubleshooting

• Reduces reagent use and sample handling time