Rapid, convenient, accurate antibody quantification
Whether you are optimising the assessment of your transient expression, accelerating your cell line development programme, or streamlining process development, frequent, accurate and efficient quantification of antibody titre is crucial.
Quickly select stable high-producing clones and optimise process conditions with the agility of highly accurate and convenient antibody quantification using Amperia Redox Electrochemical Detection (RED).
Quickly and accurately quantify antibody titre at your bench or bioreactor
Remove analytical delays when you need a simple antibody titre
Current quantitative methods such as Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) are often limited to centralised analytical laboratories due to their cost and complexity of operation. This can lead to analytical delays and requirements for sample storage.
Our Amperia RED system offers a robust, cost-effective, automated alternative for efficient, accurate antibody titre measurement.
Scatter plot showing the high correlation between Amperia RED and BLI mAb titre measurements. (R²= 0.9, P <0.0001).
Optimise workflows and focus on implementing your cell culture decisions
Streamline decision-making and optimise bioprocess workflows with actionable antibody titre results available conveniently to any team member, at any bench or bioreactor site, direct from unpurified samples.
- Deploy anywhere: optic-free and compact, the Amperia can be deployed wherever you need it most
- Simple-to-use: automated workflow consistency with no specialist training required.
- Rapid results: process up to 64 samples in just 1 hour
- Crude compatible: RED highly sensitive and specific direct detection enables antibody quantification from crude cell culture samples across a broad range (0.01 µg/mL up to 200 µg/mL) saving time on purification or serial dilutions.
- Accurate and precise: excellent data correlation with BLI, SPR in a recent independent study.
- Cost-effective: the all-in-one optic-free system, including built-in computing, means no calibration or maintenance required between runs and makes Amperia accessible to all laboratories. Standard 96-well microplate operation reduces specialised consumable costs.
Simplified Antibody quantification using Amperia RED
An automated ‘dip and measure’ approach uses pre-coated Protein A sensors optimised for capture of antibodies. Ideal for antibody quantification or binding assays using an antibody as the capture molecule. The assay takes place directly on the sensor tip, where the bound analyte triggers an electrical signal, which is read instantaneously by the device.
Protein A
Capture molecule
Antibody
Sample
HRP tagged antibody
Detection reagent
STEP 1
Protein A
A range of pre-coated RED-Sensors for different applications are available. In this example, the Protein A RED-Sensor is chosen.
STEP 2
Sample capture
The RED-Sensor is dipped into the well with the sample, which in this case contains a Sample Antibody of unknown concentration. These are captured by the Protein A on the surface of the RED-Sensor. Incubation can take as little as 30 seconds.
STEP 3
Detection Antibody
The RED-Sensor is moved into the next well containing the Detection Antibody tagged with Horseradish Peroxidase (HRP). RED is compatible with standard reagents from most vendors. The Detection Antibody binds to the Sample Antibody in as little as 30 seconds. One feature of RED is that the same wells can be re-used again, meaning the well containing the Detection Antibody can be used for multiple iterations of detection.
STEP 4
Electrochemical Measurement
In the final step of the measurement sequence, the RED-Sensor enters a well containing the substrate, e.g. Tetramethylbenzidine (TMB). Rather than measuring a change in colour, the RED-sensor measures the reaction between HRP and TMB electrochemically and in real-time. This entire steps takes only 10 seconds. The signal that is measured is proportional to the concentration of the Sample Antibody. Quantification is achieved using as standard curve.
STEP 5
Rinse & Repeat
After one iteration of the measurement, the sensors are automatically regenerated. A new sequence can start following the same Steps 1-3. Protein A sensors can be used for at least 10 measurement iterations or more.
Quantification of identical control samples by different measurement methods. Each bar represents a single measurement.
Efficient and accurate quantification of human antibodies in Cell Line Development
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Ready to see how Amperia could get your antibody titre answers quickly and accelerate your cell line development and process optimisation?
